0.25
0.5
0.75
1.25
1.5
1.75
2
Pseudomonas syringae pv. actinidiae, three years after its discovery in New Zealand: what have we learned about the pathogen, the disease and how to control it?
Published on 2013-10-143937 Views
In the last few years the causal agent of bacterial canker of kiwifruit, Pseudomonas syringae pv. actinidiae (Psa), has become a global pathogen of economic importance. Before 2008, this little known
Presentation
Pseudomonas syringae pv. actinidiae, tri leta po odkritju v Novi Zelandiji: kaj smo se naučili o patogenu, o bolezni in kako se jo obvladuje00:00
New Zealand09:25:06
The New Zealand kiwifruit industry in perspective ...18:18:53
Early November 2010: Few leaf spots ...38:18:41
Earlier in Italy ...45:15:19
The follow up in Italy (2009)47:16:53
Identification of Pseudomonas syringae pv actinidiae by PCR using the primers PsaF1/R260:56:01
November 2010 detection of Psa in New Zealand79:37:10
Box PCR patterns and DNA sequence of cts gene98:08:50
Box PCR patterns of Psa strains from New Zealand114:25:36
Box PCR patterns and pathogenicity of Psa strains from New Zealand122:34:31
Box PCR patterns and cts haplotypes from Psa strains from New Zealand129:44:47
Difference in virulence between biovar 3 and biovar 4 of Psa133:56:29
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 1140:29:34
Effector genes in strains of P. syringae pv. actinidiae from different geographic origins154:09:57
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 2161:56:08
Similar grouping by MLST using house keeping genes or effector genes165:15:00
Phylogeny of Psa based on Whole Genome Sequence179:14:13
November 2010 detection of biovar 3 Psa in New Zealand212:53:52
Bay of Plenty December 2010219:30:46
What is the situation today? (18 September 2013)245:24:53
New Zealand kiwifruit growing regions260:44:14
Cost of Psa in New Zealand (2012 study)270:21:45
Cost of Psa in New Zealand (2013 estimate)291:12:35
Climatic considerations322:10:29
Plant 1A: ‘Hort16A’ 3 weeks after inoculation339:56:35
Plant 1B: ‘Hort16A’ 3 weeks after inoculation352:38:53
Psa populations in cfu/g - 1363:34:29
Plant 1A: Constant humidity - 1371:54:49
Plant 1A: Constant humidity - 2379:16:57
Plant 1A: Constant humidity - 3380:30:04
Psa populations in cfu/g - 2381:22:45
Plant 1B: 48 hours high humidity401:18:51
Other contributing factors to the intensity of the New Zealand outbreak402:59:34
Epidemiology: where does Psa survive? - 1416:49:16
Epidemiology: where does Psa survive? - 2420:56:39
Survival on leaf litter (25 May – 1 September)426:40:09
Survival of Psa on symptomatic cane material Tyson et442:17:36
Unique and shared ortholog groups between biovars446:41:45
Effector complement varies considerable between Psa biovars459:29:43
Psa V-13 chromosome (biovar 3)475:28:18
ICEs are the key difference between biovar 3 isolates480:58:41
Distribution of Integrative Conjugative Elements in isolates from China500:08:58
Inoculation of lower leaf surface523:15:33
Colonisation of the stomata and bacteria exuding from stomata532:02:52
Leaf infection533:13:33
Infected cane (lenticel)544:04:20
Psa infected cane cross-section 1000x - 1557:02:44
Psa infected cane cross-section 1000x - 2567:59:17
Psa infected cane cross-section 1000x - 3572:44:37
Colonisation of the xylem by Psa574:05:36
Phylogeny of Psa based on Whole Genome Sequence578:30:04
Coronatine structural and functional homologue of jasmonic acid585:38:27
Signalling pathways in Arabidopsis588:46:19
Incidence of Psa on ‘Bruno’ seedlings treated with Methyl-Jasmonate 7 days before inoculation599:04:19
Signalling pathways in Arabidopsis618:08:11
Bruno’ seedlings treated with SA or ASM 7 days before inoculation631:27:59
Field experiments652:27:38
Disease incidence 21 days after inoculation with Psa666:17:55
In conclusion700:08:55
Untitled734:41:01