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Plasmids and virulence in E. amylovora and related pathogens
Published on 2012-05-253407 Views
Erwinia amylovora is the quarantine organism responsible for the fire blight of rcsacecus plants. The disease can affect pear tree, apple and loquat, and to diverse ornamental plants. This bacterium a
Presentation
Plasmids and virulence in E. amylovora and related pathogens00:00
IVIA - Instituto Valenciano de Investigaciones agrarias11:56:24
Lab. of Bacteriology. Plant Protection and Biotechnology Center24:24:51
Plasmids and virulence in E. amylovora and related pathogens31:06:17
The pathogen40:56:30
Symptoms of fire blight - 163:14:55
Symptoms of fire blight - 269:04:43
Plasmids in Erwinia amylovora71:53:40
Characteristics of Pea29 plasmid88:43:15
Molecular genetics involved in virulence and pathogenicity in Erwinia amylovora108:39:31
Fire blight in Spain (1995-2011)118:34:30
Characterization of spanish strains147:11:25
PCM with primers from plasmid pEA29 and chromosome161:36:53
Plasmids of strain IVIA 1614-2a and reference strainsa172:08:49
Analysis of plasmids by restriction digestion with BamHI187:39:59
Approximate size of plasmid from strain IVIA 1614-2a after restriction with BamHI194:30:13
Hybridization analyses of the plasmids196:52:14
Hybridization analyses after restriction with enzyme EcoRI203:31:48
Hybridization of total DNA of strain IVIA 1614-2a with pEA29 probe209:58:55
Characteristics of strain IVIA 1614-2a212:57:48
Growth curve of reference strains and IVIA 1614-2a226:49:54
Virulence studies: inoculation in several hosts231:58:32
Virulence in plants and immature pear: comparison with reference strains237:43:43
Study of virulence in natural E. amylovora strains devoid of the pEA29 kb plasmid - 1243:04:20
Analyses performed263:28:27
Plasmid profile of the isolates obtained in the outbreak in segovia271:06:35
Plasmid profiles of strains without pEA29 isolated in nature283:08:23
Study of virulence in natural E. amylovora strains devoid of the pEA29 kb plasmid - 2290:16:45
Labelling and curing of pEI70295:47:35
Virulence assays: inoculation in immature pear311:04:53
Effect of plasmids pEA29 and pEI70 on virulence315:37:42
Virulence of strains with mutants pEI70::Tn5 kanR introduced inoculation in immature pear345:50:05
Inoculation mutants pEI70::Tn5 kanR in immature pear350:22:55
Role of pEI70 in different strains with reduced virulence354:04:16
Remarks361:21:50
Presence of pEI70 in spanish and european isolates374:38:27
Duplex analyses of strains from european countries385:29:29
Distribution of strains with pEI70 in Europe395:54:36
Analyses of strains from other continents401:04:25
Related pathogenic Erwinia described and plasmids410:53:48
Genetic comparison of plasmid pEI70 with related bacteria435:35:33
Characteristics of the pEI70 plasmid453:02:39
Plasmids in pathogenic fruit tree Erwinia species: do they have a common function and origin?465:51:50
Plasmid content of related Erwinias486:40:09
Hybridization analyses with related Erwinias488:51:02
Restriction analysis with BamHI491:51:25
Hybridization analyses with pEA29492:28:42
Comparison494:26:34
Regions of pEPIR37 not detected in plasmids of other pahogenic Erwinia506:44:36
Effect of pEPIR37 in E. amylovora515:47:05
Inoculation analyses in immature loquat529:54:07
Geographical origin of different Erwinias533:12:23
Conclusions542:30:08
Thank you559:30:38