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Small RNA sequencing and assembly: reconstructing viral genomes from defence molecules
Published on Mar 07, 20133940 Views
RNA silencing constitutes a fundamental antiviral defence mechanism in plants in which host enzymes cut viral RNA into pieces of 20-24 nucleotides. When isolated, sequenced en mass and properly assemb
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Chapter list
siRNAsequencing and assembly: reconstructing viral genomes from defense molecules00:00
Next generation sequencing for viral diagnostics - 100:50
Next generation sequencing for viral diagnostics - 202:34
Anti-viral RNA silencing in eukaryotic organisms - 102:56
Anti-viral RNA silencing in eukaryotic organisms - 204:55
Procedure: simple!05:21
Deep sequencing of small RNAs from virus infected sweetpotato - 105:51
Deep sequencing of small RNAs from virus infected sweetpotato - 205:54
Deep sequencing of small RNAs from virus infected sweetpotato - 306:35
Deep sequencing of small RNAs from virus infected sweetpotato - 407:27
Proof of concept - 107:32
Proof of concept - 207:42
Proof of concept - 307:43
Proof of concept - 407:44
Proof of concept - 507:55
Proof of concept - 608:18
Other plant species - 108:35
Other plant species - 209:30
Other plant species - 309:49
Other plant species - 410:30
Beyond retroviruses - 110:45
Beyond retroviruses - 210:47
Beyond retroviruses - 310:48
Beyond retroviruses - 410:49
Beyond retroviruses - 510:50
Beyond retroviruses - 610:50
Beyond retroviruses - 710:51
Beyond retroviruses - 810:52
Beyond retroviruses - 910:53
Tissue specificity? - 111:26
Tissue specificity? - 211:26
Tissue specificity? - 312:00
Tissue specificity? - 412:06
Tissue specificity? - 512:09
Tissue specificity? - 612:25
Tissue specificity? - 712:40
Better assembly with faster sample preparation methods: higher sensitivity13:06
Small RNA sequencing and assembly: applications - 113:47
Small RNA sequencing and assembly: applications - 214:18
Small RNA sequencing and assembly: applications - 315:09
Small RNA sequencing and assembly: applications - 415:20
Small RNA sequencing and assembly: applications - 515:57
Small RNA sequencing and assembly: applications - 616:04
Small RNA sequencing and assembly: applications - 716:56
Pathogen variability studies: PVY & PVX17:28
Going to scale: the pan African sweetpotato virome19:07
Recorded data - 119:53
Recorded data - 220:52
Mozambique20:54
Virus variation23:28
Ghana & Burkina Faso24:48
What to do with this? - 126:10
What to do with this? - 226:36
Small RNA sequencing and assembly: lessons learnt26:59
Special thanks too29:16